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LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) <t>Serum</t> <t>H-FABP</t> levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.
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LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) <t>Serum</t> <t>H-FABP</t> levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.
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LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) <t>Serum</t> <t>H-FABP</t> levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.
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LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) <t>Serum</t> <t>H-FABP</t> levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.
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Diagram and components of the lateral flow immunoassay test strip <t>for</t> <t>H-FABP</t> (A) and visual assessment guidelines for interpreting the test strip results (B) . Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.
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LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.

Journal: Frontiers in Pharmacology

Article Title: Levocarnitine improves cardiac energy metabolic remodeling in myocarditis mice

doi: 10.3389/fphar.2025.1706936

Figure Lengend Snippet: LC improves myocardial mitochondrial substrate utilization in EAM mice. (A) Serum H-FABP levels in each group of mice, n = 3. (B–D) Western blot analysis of OCTN-2 and CPT-1B proteins, n = 3. (E) FFA and (F) LAC levels in myocardial tissue from each group of mice, n = 3. Data are expressed as Mean ± SEM with individual data points shown. FABP, heart-type fatty acid-binding protein; OCTN2, organic carnitine transporter novel type 2; CPT-1B, carnitine palmitoyltransferase-1B; FFA, free fatty acid; LAC, lactic acid.

Article Snippet: Follow the instructions in the H-FABP ELISA kit (JM-04323H2, Jingmei, Jiangsu, China) manual to determine H-FABP levels in each group’s serum using a multifunctional enzyme analyzer (Molecular Devices, Shanghai, China).

Techniques: Western Blot, Binding Assay

Diagram and components of the lateral flow immunoassay test strip for H-FABP (A) and visual assessment guidelines for interpreting the test strip results (B) . Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: Diagram and components of the lateral flow immunoassay test strip for H-FABP (A) and visual assessment guidelines for interpreting the test strip results (B) . Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques: Stripping Membranes, Binding Assay

Characteristion, optimal pH and labelling concentration of the SeNps-anti-H-FABP mAb1. TEM (A) and DLS (B) result of SeNps-anti-H-FABP mAb; Detection results and auxiliary judgement result (C) with H-FABP kit at different pH values; Detection results and auxiliary judgement result (D) with H-FABP kit at different concentrations of mAb1.

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: Characteristion, optimal pH and labelling concentration of the SeNps-anti-H-FABP mAb1. TEM (A) and DLS (B) result of SeNps-anti-H-FABP mAb; Detection results and auxiliary judgement result (C) with H-FABP kit at different pH values; Detection results and auxiliary judgement result (D) with H-FABP kit at different concentrations of mAb1.

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques: Concentration Assay

The limit of detection of the H-FABP kit in plasma and blood. Detection results for H-FABP in plasma observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) . Detection results for H-FABP in blood observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D) .

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: The limit of detection of the H-FABP kit in plasma and blood. Detection results for H-FABP in plasma observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) . Detection results for H-FABP in blood observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D) .

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques:

The specificity and stability of the H-FABP kit. Detection results of specificity for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) . Detection results of stability for H-FABP observed by the naked eyes and auxiliary interpretation results by ImageJ and GraphPad Prism (C) . Abbreviations: H-FABP: Heart-type fatty acid binding protein; cTnI: Cardiac troponin I; Myo: Myoglobin; CK-MB: Creatine kinase-Mb; C: Control line; T: Test line.

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: The specificity and stability of the H-FABP kit. Detection results of specificity for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) . Detection results of stability for H-FABP observed by the naked eyes and auxiliary interpretation results by ImageJ and GraphPad Prism (C) . Abbreviations: H-FABP: Heart-type fatty acid binding protein; cTnI: Cardiac troponin I; Myo: Myoglobin; CK-MB: Creatine kinase-Mb; C: Control line; T: Test line.

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques: Binding Assay

Results of Recovery Experiment for H-FABP

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: Results of Recovery Experiment for H-FABP

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques:

Detection results of actual samples of this kits for H-FABP in plasma and blood samples. Detection results of plasma for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) ; Detection results of blood for H-FABP observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D) .

Journal: International Journal of Nanomedicine

Article Title: Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

doi: 10.2147/IJN.S359541

Figure Lengend Snippet: Detection results of actual samples of this kits for H-FABP in plasma and blood samples. Detection results of plasma for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B) ; Detection results of blood for H-FABP observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D) .

Article Snippet: The H-FABP ELISA Kit was obtained from Hycult Biotech (Catalog number: HK401; Lot number: 19750K0517-Z).

Techniques: